BLAST - Basic Local Alignment Search Tool

BLAST finds regions of similarity between biological sequences. The program compares nucleotide or protein sequences to sequence databases and calculates the statistical significance.

Update notes

BLAST+ 2.13.0 is here!

Thu, 17 Mar 2022 12:00:00 EST

Starting with this release, we are including the blastn_vdb and tblastn_vdb executables in the BLAST+ distribution. These executables can directly search SRA and WGS projects without the need to build a BLAST database. See https://ftp.ncbi.nlm.nih.gov/blast/WGS_TOOLS/README_BLASTWGS.txt for information on using these executables with WGS projects.

We have also produced ARM LINUX executables and placed them on the FTP site (ncbi-blast-2.13.0+-x64-arm-linux.tar.gz). These executables have passed our normal QA, but this is our first release for this architecture. Please let us know if you find any issues.

Usage reporting - Help improve BLAST by sharing limited information about your search. Details on the information collected, how it is used, how to opt-out, and our privacy statement is found here: https://www.ncbi.nlm.nih.gov/books/NBK563686/

rpsbproc is included

rpsbproc allows for filtering of rpsblast searches - use the databases in /nfs1/CGRB/databases/NCBI/cdd/latest for this purpose.

Location and version:

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$ which blastn
/local/cluster/ncbi-blast+/bin/blastn
$ blastn -version
blastn: 2.13.0+
 Package: blast 2.13.0, build May 13 2022 11:32:40

For help, see the relevant blast executable.

software ref: https://blast.ncbi.nlm.nih.gov/Blast.cgi
software ref: https://blast.ncbi.nlm.nih.gov/Blast.cgi?CMD=Web&PAGE_TYPE=BlastNews
research ref: https://doi.org/10.1186/1471-2105-10-421