Falco: FastQC Alternative Code
This program is an emulation of the popular FastQC software to check large
sequencing reads for common problems.
Location and version:
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$ which falco
/local/cluster/bin/falco
$ falco --version
falco 0.3.0
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help message:
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$ falco --help
Usage: falco [OPTIONS] <seqfile1> <seqfile2> ...
Options:
-h, --help print this help file and exit
-v, --version print the program version and exit
-o, --outdir Create all output files in the specified
output directory. If notprovided, files
will be created in the same directory as
the input file.
-C, --casava Files come from raw casava output
(currently ignored)
-n, --nano Files come from fast5 nanopore sequences
-F, --nofilter If running with --casava do not sequences
(currently ignored)
-e, --noextract If running with --casava do not remove poor
quality sequences (currently ignored)
-g, --nogroup Disable grouping of bases for reads >50bp
-f, --format Force file format
-t, --threads Specifies number of threads to process
simultaneos files in parallel (currently
set for compatibility with fastqc. Not yet
supported!)
-c, --contaminants Non-default filer with a list of
contaminants
-a, --adapters Non-default file with a list of adapters
-l, --limits Non-default file with limits and warn/fail
criteria
-T, --skip-text Skip generating text file (Default = false)
-H, --skip-html Skip generating HTML file (Default = false)
-S, --skip-short-summary Skip short summary(Default = false)
-q, --quiet print more run info
-d, --dir directory in which to create temp files
-A, --advanced-mode advanced mode: adds more information to the
FastQC output depending on non-fastqc user
flags
-B, --bisulfite reads are whole genome bisulfite
sequencing, and more Ts and fewer Cs are
therefore expected and will be accounted
for in base content (advanced mode)
-R, --reverse-complement The input is a reverse-complement. All
modules will be tested by swapping A/T and
C/G
Help options:
-?, -help print this help message
-about print about message
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falco should result in a speed-up over FASTQC but is still not completely a 1:1
replacement. Keep development in mind when using FASTQC.
software ref: https://github.com/smithlabcode/falco
research ref: https://doi.org/10.12688/f1000research.21142.2